请选择 进入手机版 | 继续访问电脑版

二次谐波成像

已有 106 次阅读2021-2-24 01:26 |个人分类:医学影像|系统分类:医学科学

二次谐波成像。正如史蒂文·佩吉在他的“种子和土壤假说”中首次提出的那样,理解肿瘤的进展不仅涉及研究肿瘤细胞本身,还涉及研究肿瘤周围的细胞和结构。因此,研究肿瘤进展过程中的基质变化对于理解可能导致转移增加的微环境变化至关重要。目前监测肿瘤进展的临床方法往往无法以足够的时间和空间分辨率监测间质变化:HE或免疫组化染色组织切片的成像提供了肿瘤中发生的间质变化的高分辨率图像,但只能及时提供肿瘤进展的“快照”。其他的活体成像方法,如正电子发射断层摄影术(PET)、磁共振成像(MRI)或超声波,在过去几年中显著提高了肿瘤的检出率,并可以监测时间变化,但缺乏空间分辨率和/或分子敏感度。活体显微镜,特别是多光子显微镜填补了这些技术之间的空白。
As first suggested by Steven Paget with his “Seed and Soil Hypothesis,” understanding tumor progression not only involves studying the tumor cells themselves, but also the cells and structures surrounding them. Consequently, studying the stromal changes throughout tumor progression is critical for under standing microenvironmental changes that may lead to increased metastasis. Current clinical methods of monitoring tumor progression are often incapable of monitoring stromal changes with adequate temporal and spatial resolution: Imaging of H&E or immunohistochemically stained tissue sections provides high resolution visualization of stromal changes occurring in the tumor, but only provides a “snapshot” of tumor progression in time. Other methods of intravital imaging, such as Positron Emission Tomogrophy (PET), Magnetic Resonance Imaging (MRI), or ultrasound have improved tumor detection significantly over the course of the last few years, and can monitor temporal changes,3-5 but lack spatial resolution and/or molecular sensitivity.The gap existing between these techniques is filled by intravital microscopy and especially multiphoton microscopy (MPM).

路过

鸡蛋

鲜花

握手

雷人

发表评论 评论 (1 个评论)

回复 hyc3140 2021-2-24 01:27
Burke K, Brown E. The Use of Second Harmonic Generation to Image the Extracellular Matrix During Tumor Progression[J]. IntraVital, 2015,3:e984509.

facelist

您需要登录后才可以评论 登录 | 立即注册

返回顶部